Regarding Flash memory map

Modifing the FLASH memory allocation for Backup Image, File System Size and Application image from
Options/NET+OS /Flash MemoryMap

requires rom.bin to be uploaded to the Connect ME 9210 module?

no, it should not be required. rom.bin unless modified itself will always occupy the first sector in FLASH and will always look for image.bin from the first address of image.bin. The reset of the memory map does not concern bootloader.